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KMID : 0380020030180050404
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 5 p.404 ~ p.407
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Synthesis of Poly[3-hydroxybutyrate-co-3-hydroxyvalerate] by Recombinant Escherichia coli from Whey
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Kim Beom-Soo
Lee Sang-Yup
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Abstract
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Two recombinant Escherichia coli strains, GCSC6576 harboring a plasmid pSYL107 containing the Ralstonia eutropha polyhydroxyalkanoate (PHA) biosynthesis genes and a fadR atoC mutant LS5218 harboring a plasmid pJC4 containing the Alcaligenes latus PHA biosynthesis genes were compared for their ability to synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV) from whey. The 3HV fraction could be increased by acetic acid induction and oleic acid supplementation in flask cultures of recombinant E. coli GCSC6576. With the pH-stat fed-batch culture of recombinant E. coli LS5218, we obtained a cell concentration, a P(3HB-co-3HV) concentration, a P(3HB-co-3HV) content, and a 3HV fraction of 31.8 g/L, 10.6 g/L, 33.4%, and 6.26 mol%, respectively in 39 h.
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KEYWORD
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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate), recombinant Escherichia coli, whey, fed-batch culture
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